A multiplicity of CCAAT box-binding proteins
Identifieur interne : 000415 ( France/Analysis ); précédent : 000414; suivant : 000416A multiplicity of CCAAT box-binding proteins
Auteurs : Arnulf Dorn [France] ; Jacques Bollekens [France] ; Adrian Staub [France] ; Christophe Benoist [France] ; Diane Mathis [France]Source :
- Cell [ 0092-8674 ] ; 1987.
English descriptors
- Teeft :
- Antisense, Antisense strand, Antisense strands, Arrows point, Assay, Band intensity, Binding, Binding core, Binding efficiency, Binding properties, Binding proteins, Binding reaction, Binding requirements, Binding site, Biol, Ccaat, Ccaat boxes, Ccaat protein, Ccaat proteins, Ccaat sequence, Ccaat sequences, Chicken liver, Cleavage, Cleavage products, Competition experiments, Complex formation, Contact sites, Different entities, Distinct proteins, Dna, Dnaase, Dorn, Element harbors, Ethanol precipitation, Experimental evidence, Fetal haemoglobin, Fragment, Functional relevance, Gene, Gene expression, Gene promoters, Good correlation, Heat shock transcription factor, Hela, Hela cell, Hela cells, Kelly, Major histocompatibility, Mcknight, Methylated, Methylation, Methylation interference, Methylation interference mapping, Mouse promoter, Murine, Murine class, Mutagenesis, Mutation, Neutral polyacrylamide, Nuclear protein, Nuclear proteins, Nucleotide, Oligo, Oligonucleotide, Oligonucleotides, Oligos, Other hand, Other oligonucleotides, Point mutation, Polyacrylamide, Promoter, Promoter fragment, Promoter function, Promoter region, Purine contacts, Random sequence, Relative binding efficiencies, Retardation, Retardation assay, Retardation assays, Retarded band, Room temperature, Same protein, Saturation mutagenesis, Saturation mutagenesis experiment, Sense strand, Sequence elements, Sequencing, Specific binding, Thymidine kinase promoter, Tjian, Transcription, Transcription factor, Transcriptional, Transcriptional control elements, Typical binding reaction, Unlabeled, Unlabeled competitor oligonucleotide.
Abstract
Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.
Url:
DOI: 10.1016/0092-8674(87)90513-7
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002511
- to stream Istex, to step Curation: 002511
- to stream Istex, to step Checkpoint: 002189
- to stream Main, to step Merge: 004D89
- to stream Main, to step Curation: 004D09
- to stream Main, to step Exploration: 004D09
- to stream France, to step Extraction: 000415
Links to Exploration step
ISTEX:523F85199F114B4B17AEEAF87EFEBEFCCE27C897Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>A multiplicity of CCAAT box-binding proteins</title><author><name sortKey="Dorn, Arnulf" sort="Dorn, Arnulf" uniqKey="Dorn A" first="Arnulf" last="Dorn">Arnulf Dorn</name></author><author><name sortKey="Bollekens, Jacques" sort="Bollekens, Jacques" uniqKey="Bollekens J" first="Jacques" last="Bollekens">Jacques Bollekens</name></author><author><name sortKey="Staub, Adrian" sort="Staub, Adrian" uniqKey="Staub A" first="Adrian" last="Staub">Adrian Staub</name></author><author><name sortKey="Benoist, Christophe" sort="Benoist, Christophe" uniqKey="Benoist C" first="Christophe" last="Benoist">Christophe Benoist</name></author><author><name sortKey="Mathis, Diane" sort="Mathis, Diane" uniqKey="Mathis D" first="Diane" last="Mathis">Diane Mathis</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:523F85199F114B4B17AEEAF87EFEBEFCCE27C897</idno><date when="1987" year="1987">1987</date><idno type="doi">10.1016/0092-8674(87)90513-7</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-J69FD1W8-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002511</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002511</idno><idno type="wicri:Area/Istex/Curation">002511</idno><idno type="wicri:Area/Istex/Checkpoint">002189</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002189</idno><idno type="wicri:doubleKey">0092-8674:1987:Dorn A:a:multiplicity:of</idno><idno type="wicri:Area/Main/Merge">004D89</idno><idno type="wicri:Area/Main/Curation">004D09</idno><idno type="wicri:Area/Main/Exploration">004D09</idno><idno type="wicri:Area/France/Extraction">000415</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">A multiplicity of CCAAT box-binding proteins</title><author><name sortKey="Dorn, Arnulf" sort="Dorn, Arnulf" uniqKey="Dorn A" first="Arnulf" last="Dorn">Arnulf Dorn</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Bollekens, Jacques" sort="Bollekens, Jacques" uniqKey="Bollekens J" first="Jacques" last="Bollekens">Jacques Bollekens</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Staub, Adrian" sort="Staub, Adrian" uniqKey="Staub A" first="Adrian" last="Staub">Adrian Staub</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Benoist, Christophe" sort="Benoist, Christophe" uniqKey="Benoist C" first="Christophe" last="Benoist">Christophe Benoist</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Mathis, Diane" sort="Mathis, Diane" uniqKey="Mathis D" first="Diane" last="Mathis">Diane Mathis</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">50</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="863">863</biblScope><biblScope unit="page" to="872">872</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antisense</term><term>Antisense strand</term><term>Antisense strands</term><term>Arrows point</term><term>Assay</term><term>Band intensity</term><term>Binding</term><term>Binding core</term><term>Binding efficiency</term><term>Binding properties</term><term>Binding proteins</term><term>Binding reaction</term><term>Binding requirements</term><term>Binding site</term><term>Biol</term><term>Ccaat</term><term>Ccaat boxes</term><term>Ccaat protein</term><term>Ccaat proteins</term><term>Ccaat sequence</term><term>Ccaat sequences</term><term>Chicken liver</term><term>Cleavage</term><term>Cleavage products</term><term>Competition experiments</term><term>Complex formation</term><term>Contact sites</term><term>Different entities</term><term>Distinct proteins</term><term>Dna</term><term>Dnaase</term><term>Dorn</term><term>Element harbors</term><term>Ethanol precipitation</term><term>Experimental evidence</term><term>Fetal haemoglobin</term><term>Fragment</term><term>Functional relevance</term><term>Gene</term><term>Gene expression</term><term>Gene promoters</term><term>Good correlation</term><term>Heat shock transcription factor</term><term>Hela</term><term>Hela cell</term><term>Hela cells</term><term>Kelly</term><term>Major histocompatibility</term><term>Mcknight</term><term>Methylated</term><term>Methylation</term><term>Methylation interference</term><term>Methylation interference mapping</term><term>Mouse promoter</term><term>Murine</term><term>Murine class</term><term>Mutagenesis</term><term>Mutation</term><term>Neutral polyacrylamide</term><term>Nuclear protein</term><term>Nuclear proteins</term><term>Nucleotide</term><term>Oligo</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Oligos</term><term>Other hand</term><term>Other oligonucleotides</term><term>Point mutation</term><term>Polyacrylamide</term><term>Promoter</term><term>Promoter fragment</term><term>Promoter function</term><term>Promoter region</term><term>Purine contacts</term><term>Random sequence</term><term>Relative binding efficiencies</term><term>Retardation</term><term>Retardation assay</term><term>Retardation assays</term><term>Retarded band</term><term>Room temperature</term><term>Same protein</term><term>Saturation mutagenesis</term><term>Saturation mutagenesis experiment</term><term>Sense strand</term><term>Sequence elements</term><term>Sequencing</term><term>Specific binding</term><term>Thymidine kinase promoter</term><term>Tjian</term><term>Transcription</term><term>Transcription factor</term><term>Transcriptional</term><term>Transcriptional control elements</term><term>Typical binding reaction</term><term>Unlabeled</term><term>Unlabeled competitor oligonucleotide</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Strasbourg</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Dorn, Arnulf" sort="Dorn, Arnulf" uniqKey="Dorn A" first="Arnulf" last="Dorn">Arnulf Dorn</name></region><name sortKey="Benoist, Christophe" sort="Benoist, Christophe" uniqKey="Benoist C" first="Christophe" last="Benoist">Christophe Benoist</name><name sortKey="Bollekens, Jacques" sort="Bollekens, Jacques" uniqKey="Bollekens J" first="Jacques" last="Bollekens">Jacques Bollekens</name><name sortKey="Mathis, Diane" sort="Mathis, Diane" uniqKey="Mathis D" first="Diane" last="Mathis">Diane Mathis</name><name sortKey="Staub, Adrian" sort="Staub, Adrian" uniqKey="Staub A" first="Adrian" last="Staub">Adrian Staub</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000415 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000415 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:523F85199F114B4B17AEEAF87EFEBEFCCE27C897
|texte= A multiplicity of CCAAT box-binding proteins
}}
| This area was generated with Dilib version V0.6.33. |  |